Oocyte activation, phospholipase C zeta and human infertility
نویسندگان
چکیده
منابع مشابه
A maternally inherited autosomal point mutation in human phospholipase C zeta (PLCζ) leads to male infertility.
BACKGROUND Male factor and idiopathic infertility contribute significantly to global infertility, with abnormal testicular gene expression considered to be a major cause. Certain types of male infertility are caused by failure of the sperm to activate the oocyte, a process normally regulated by calcium oscillations, thought to be induced by a sperm-specific phospholipase C, PLCzeta (PLCζ). Prev...
متن کاملPhospholipase Cζ rescues failed oocyte activation in a prototype of male factor infertility
OBJECTIVE To determine the effect of infertility-linked sperm phospholipase Cζ (PLCζ) mutations on their ability to trigger oocyte Ca(2+) oscillations and development, and also to evaluate the potential therapeutic utility of wild-type, recombinant PLCζ protein for rescuing failed oocyte activation and embryo development. DESIGN Test of a novel therapeutic approach to male factor infertility....
متن کاملP-84: Phospholipase C Zeta: As an Index to Assess Fertilization Potential of a Semen Sample
Background: Failed fertilization post ICSI has been mainly attributed to the sperm’s inability to induce oocyte activation. Phospholipase C Zeta (PLCζ) is considered to be a primary factor for the induction of oocyte activation. The aim of this study was to quantitatively assess the expression of PLCζ in globozoospermic or individuals with previous low or failed fertilization in comparison to f...
متن کاملO-15: Reduced Fertilization After ICSI and Abnormal Phospholipase C Zeta Presence in Spermatozoa from the Wobbler Mouse
Background: Failed fertilization after intracytoplasmic sperm injection (ICSI) can be due to a reduced oocyte-activation capacity caused by reduced concentrations and abnormal localization of the oocyte-activation factor phospholipase C (PLC) zeta. Patients with this condition can be helped to conceive by artificial activation of oocytes after ICSI with calcium ionophore (assisted oocyte activa...
متن کاملRelationship between phospholipase C zeta immunoreactivity and DNA fragmentation and oxidation in human sperm
OBJECTIVE The study aimed to evaluate the feasibility and reproducibility of measuring phospholipase C zeta (PLCζ) using immunostaining in human sperm and to investigate the relationship between PLCζ immunoreactivity and DNA fragmentation and oxidation in human sperm. METHODS Semen samples were obtained from participants (n=44) and processed by the conventional swim-up method. Sperm concentra...
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ژورنال
عنوان ژورنال: Human Reproduction Update
سال: 2010
ISSN: 1355-4786,1460-2369
DOI: 10.1093/humupd/dmq018